the hedgehog/gli-1 signaling pathways is involved in the inhibitory effect of resveratrol on human colorectal cancer hct116 cells

objective(s): the study aimed to investigate the effects of resveratrol on colorectal cancer hct116 cells, including cell viability, apoptosis, and migration, and the partial mechanisms focused on hedgehog/gli-1 signaling pathways. materials and methods: we chose the appropriate time and concentration of recombinant human sonic hedgehog (shh) stimulation by cell viability. the proportion of cell apoptosis was detected by flow cytometry; hct116 cell migration was measured by scratch test; the expression of ptch, smo, and gli-1 was measured by western blot analysis. results: shh signaling increased hct116 cell viability and migration, inhibited cell apoptosis, and upregulated the expression of ptch, smo, and gli-1. resveratrol obviously inhibited hct116 cell viability and migration, promoted cell apoptosis, and suppressed the protein of ptch, smo, and gli-1. furthermore, the effects of resveratrol and shh on human colorectal cancer hct116 cells were in a dose- and time-dependent manner. conclusion: the inhibitory effect of resveratrol on hct116 cells may be mediated by hedgehog/gli-1 signaling pathways.